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KMID : 1134820160450111580
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 11 p.1580 ~ p.1588
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Antioxidative and Neuroprotective Effects of Extract and Fractions from Adenophora triphylla
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Chung Mi-Ja
Lee Sang-Hyun
Park Yong-Il
Kwon Ki-Han
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Abstract
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The 70% ethanol extract from Adenophora triphylla showed a strong antioxidant effect and reduced cytotoxicity of H2O2 in SK-N-SH cells. The chloroform fraction from A. triphylla extract (AT-CH) among the six fractions showed strong DPPH radical and intracellular reactive oxygen species (ROS) scavenger effects and the highest protective effect against H2O2-induced SK-N-SH cell death. Bioactivity compounds were purified from AT-CH, and the chemical structures of the compounds were determined as β-sitosterol and daucosterol on the basis of 1H-NMR, 13C-NMR, and EI mass spectra. β-Sitosterol and daucosterol also had protective effects against oxidative stress in SK-N-SH cells. Phospho-p38 MAPK levels were elevated by H2O2 but were inhibited by treatment with AT-CH and phytosterols (β-sitosterol and daucosterol) isolated from AT-CH. These results suggest that AT-CH has brain neuroprotective effects against oxidative stress (H2O2) by inhibiting activation of p38 pathways and scavenging intracellular ROS.
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KEYWORD
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Adenophora triphylla, antioxidant effect, neuroprotective effects, oxidative stress, SK-N-SH cells
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